Photo: Lotta Eriksson
Part of text written by Magdalena Lindén for “CMM news”:
I introduced CRISPR in discussions organized by Swedish National Council on Medical Ethics
Author: fredrikwermeling
We are very grateful for the generous grant from Vetenskapsrådet (Swedish Research Council)
The project “STUDIES OF NEUTROPHILS IN AUTOIMMUNE JOINT INFLAMMATION” was generously funded 2021-2024!
https://www.vr.se/soka-finansiering/beslut/2021-08-25-medicin-och-halsa.html
Yunbing´s paper is out!
I’m delighted to share the work led by Yunbing Shen, with help from especially Long Jiang and Vaishnavi Iyer.
“A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system”
Paper: https://tinyurl.com/4sedjhtm
Linked soundtrack: https://tinyurl.com/5465m73y
CRISPR never generates a uniform genotype which complicates analyzing the targeted gene’s role without establishing clonal lines. Here we give examples of how the generated genetic heterogeneity can be leveraged for discovery –> RCC!
See Twitter threed for more info –> https://twitter.com/Wermeling_lab/status/1452537209320583173
Seraching for the perfect gRNA (Xiang X et al)!
Very interesting study from Xiang X et al. about gRNA efficiency prediction, including a web based software CRISPRon for gRNA design (https://rth.dk/resources/crispr/crispron/).
BAR-Seq clonal tracking of gene-edited cells!
Beautiful work from Ferrari et al!
https://www.nature.com/articles/s41596-021-00529-x
Linked webpage for analysis:
Popular scientific texts and interviews
2024 / Interview in The Scientist about a CRISPR screen study, link:
2018 / Text (Swedish) for the membership magazine Legatus Mensae (“A strong Immune System”):
2021 / Interview for CMM news (text by Magdalena Lindén) :
2021 / Interview for Nordic Life Science about the Nobel Prize in Chemistry for CRISPR (p. 38-43 here in English):
2020 / Interview for KI about the Nobel Prize in Chemistry for CRISPR (here in Swedish):
2020 / Interview for Reumatikervärlden, the membership magazine of the Swedish Rheumatism Association (in Swedish). Rumatikervärlden number 2 2020 page 36-37 (interview by Annika Sjöberg):
2005 / Text for the student magazine “Bukpressen”:
I was interview about the CRISPR Nobel prize!
New paper out by Sanjay!
Great work by Sanjay Boddul in the lab developing methods to study T cell receptors cloned from patients! The presented experimental pipeline enables rapid development of relevant humanized animal models for studying novel antigen-specific tolerance therapeutics.
Boddul SV, Sharma RK, Dubnovitsky A, Raposo B, Gerstner C, Shen Y, Iyer VS, Kasza Z, Kwok WW, Winkler AR, Klareskog L, Malmström V, Bettini M, Wermeling F.
In vitro and ex vivo functional characterization of human HLA-DRB1*04 restricted T cell receptors.
J Transl Autoimmun. 2021 March (Accepted). link
3000 free medical images
The Immune Epitope Database!
The Immune Epitope Database (IEDB) is a freely available resource funded by NIAID. It catalogs experimental data on antibody and T cell epitopes studied in humans, non-human primates, and other animal species in the context of infectious disease, allergy, autoimmunity and transplantation. The IEDB also hosts tools to assist in the prediction and analysis of epitopes.
The Wermeling lab 