“We present ampliCan, an analysis tool that unites identification, quantification and visualization of genuine genome editing events from CRISPR amplicon sequencing data. ampliCan overcomes methodological challenges suffered by other tools to estimate the true mutational efficiency in a high-throughput automated fashion. ampliCan controls for biases at every step of the analysis and generates reports that allow users to quickly identify successful editing events or potential issues with their experiments. We benchmarked ampliCan against other leading tools demonstrating that it outperformed all in the face of common confounding factors.”
Highly recommended review regarding CRISPR screens:
Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art instrumentation or the need for years of hands-on experience, for better or worse, ensures slow adoption by the community. CRISPR technology, however, presents the opposite dilemma, where the simplicity of the system enabled the parallel development of many applications, improvements and derivatives, and new users are now presented with an almost paralyzing abundance of choices. This Review intends to guide users through the process of applying CRISPR technology to their biological problems of interest, especially in the context of discovering gene function at scale.
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