The Wermeling lab at the Karolinska Institutet
– Research related to Autoimmunity, Cancer immunotherapy, and CRISPR/Cas9
We are super proud of this publication. See Twitter thread for more info: https://twitter.com/Wermeling_lab/status/1462857299073114119
https://pubmed.ncbi.nlm.nih.gov/34750099/
KI press release featured on the KI.se first page:
https://news.ki.se/new-findings-on-the-link-between-crispr-gene-editing-and-mutated-cancer-cells
Photo: Lotta Eriksson
Part of text written by Magdalena Lindén for “CMM news”:
The project “STUDIES OF NEUTROPHILS IN AUTOIMMUNE JOINT INFLAMMATION” was generously funded 2021-2024!
https://www.vr.se/soka-finansiering/beslut/2021-08-25-medicin-och-halsa.html
I’m delighted to share the work led by Yunbing Shen, with help from especially Long Jiang and Vaishnavi Iyer.
“A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system”
Paper: https://tinyurl.com/4sedjhtm
Linked soundtrack: https://tinyurl.com/5465m73y
CRISPR never generates a uniform genotype which complicates analyzing the targeted gene’s role without establishing clonal lines. Here we give examples of how the generated genetic heterogeneity can be leveraged for discovery –> RCC!
See Twitter threed for more info –> https://twitter.com/Wermeling_lab/status/1452537209320583173
Very interesting study from Xiang X et al. about gRNA efficiency prediction, including a web based software CRISPRon for gRNA design (https://rth.dk/resources/crispr/crispron/).
Beautiful work from Ferrari et al!
https://www.nature.com/articles/s41596-021-00529-x
Linked webpage for analysis:
2018 / Text (Swedish) for the membership magazine Legatus Mensae (“A strong Immune System”):
2021 / Interview for CMM news (text by Magdalena Lindén) :
2021 / Interview for Nordic Life Science about the Nobel Prize in Chemistry for CRISPR (p. 38-43 here in English):
2020 / Interview for KI about the Nobel Prize in Chemistry for CRISPR (here in Swedish):
2020 / Interview for Reumatikervärlden, the membership magazine of the Swedish Rheumatism Association (in Swedish). Rumatikervärlden number 2 2020 page 36-37 (interview by Annika Sjöberg):
2005 / Text for the student magazine “Bukpressen”:
Great work by Sanjay Boddul in the lab developing methods to study T cell receptors cloned from patients! The presented experimental pipeline enables rapid development of relevant humanized animal models for studying novel antigen-specific tolerance therapeutics.
Boddul SV, Sharma RK, Dubnovitsky A, Raposo B, Gerstner C, Shen Y, Iyer VS, Kasza Z, Kwok WW, Winkler AR, Klareskog L, Malmström V, Bettini M, Wermeling F.
In vitro and ex vivo functional characterization of human HLA-DRB1*04 restricted T cell receptors.
J Transl Autoimmun. 2021 March (Accepted). link
The Wermeling lab at the Karolinska Institutet 