Using experimental systems and patient material we study the immune system with specific interest related to hematopoesis/leukemia, autoimmune disease, and cancer therapy.
A major part of the different projects in the lab involves using CRISPR/Cas9 based custom screens as a discovery platform to understand complex biological processes and to identify novel drug targets. To facilitate the design of these custom screens, we have developed a freely accessible web-based software that we call Green Listed (Panda et al, 2017, Bioinformatics) that can be accessed here. A blog post describing our approach can be found at Addgene (here). A recent review describing bioinformatic tools we use designing and analyzing hypothesis-driven CRISPR screens can be found here (Iyer et al, 2020). We also continuously publish videos on YouTube describing how we work with CRISPR and CRISPR screens on our YouTube channel.
Currently, several projects in the lab relate to our interest in signaling downstream of the IL-4 receptor (IL-4R). The basis for this interest was the unexpected finding that the expression of the IL-4R is essential for the activity of a drug (IVIG) used to treat patients with autoimmune disease and that the expression of this receptor is highly regulated during inflammation (Anthony et al, 2011, Nature and Wermeling et al, 2013, PNAS). Recently we published a study describing how IL-4 can affect neutrophils, and consequences of this in the autoimmune setting (Panda et al, 2020, PNAS)
Other projects in the lab are using in vivo CRISPR screens to identify central genes affecting the behavior of hematopoietic stem cells, T cells and neutrophils in autoimmune disease and cancer.
The Wermeling Laboratory is located at the Center for Molecular Medicine (CMM), Karolinska University Hospital, Solna, Sweden, physically located here. The group is part of the Rheumatology Unit, Department of Medicine, Karolinska Institutet, Solna.
This website is maintained by Fredrik Wermeling. Opinions expressed are the author’s own and do not necessarily reflect the view of the Karolinska Institutet, the Karolinska Hospital, CMM or any other organization mentioned on the website.