Green Listed is a tool facilitating the rapid design of custom CRISPR screens. It is used to mine publicly available source lists/reference libraries of gRNAs (see examples below) to design custom libraries targeting genes specified by the user, such as all kinases or all genes up-regulated in an RNAseq experiment.
Green Listed can be accessed through “greenlisted.cmm.ki.se” (here).
The publication: Green Listed – A CRISPR Screen Tool. Sudeepta Kumar Panda, Sanjay V. Boddul, Guillermina Yanek Jiménez-Andrade, Long Jiang, Zsolt Kasza, Luciano Fernandez-Ricaud, Fredrik Wermeling. Bioinformatics, 22 December 2016, doi: 10.1093/bioinformatics/btw739. Link
Several videos describing different parts of the software can be found on our youtube channel:
The Wermeling lab at the Karolinska Institutet 