The Wermeling lab at the Karolinska Institutet
– Research related to Autoimmunity, Cancer immunotherapy, and CRISPR/Cas9
Design and synthesis of a minimal bacterial genome.
Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC.
Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253.
A special issue explores what it means to be living in an age of gene editing.
Illustration by Chris Labrooy
“The Swedish Rheumatism Association [Svenska Reumatikerförbundet] is a non-profit organisation working for people with rheumatic disorders.” link
“Stiftelsen skapades 1953 genom en donation av dåvarande överintendenten Åke Wiberg. Den har under sin existens gett anslag till vetenskaplig forskning och till vård och utbildning av barn och ungdom. Av hävd har Stiftelsen framför allt stött medicinsk forskning men även forskning inom humaniora och samhällsvetenskap.” link
These are two fantastic resources for guide design and CRISPR screens; taking both on-target and off-target activity into account when designing guides!
We are curious how the novel algorithms are going to perform related to modified Cas9 versions such as the eSpCas9(1.1) recently described by Slaymaker et al. (Science 2016. link).
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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench et al. Nature Biotechnology 2016. link
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari et al. Nature Methods 2015. link
The Wermeling lab at the Karolinska Institutet 