TIDE –> Identification of Indel frequencies in a CRISPR targeted cell population

TIDE: Tracking of Indels by DEcomposition

TIDE is a web tool which rapidly assesses genome editing of a target locus by CRISPR-Cas9. Based on the quantitative sequence trace data from two standard capillary (Sanger) sequencing reactions, the TIDE software quantifies editing efficacy and identifies the predominant types of insertions and deletions (indels) in the DNA of a targeted cell pool. See Brinkman et al. 2014 Nucl. Acids Res.for a detailed explanation and examples.


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