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The Wermeling lab at the Karolinska Institutet

– Research related to Autoimmunity, Cancer immunotherapy and CRISPR/Cas9

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Addgene just published a blog post I´ve written about how we work with custom CRISPR screens!

http://blog.addgene.org/custom-crispr-screens-the-green-listed-software

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Author fredrikwermelingPosted on July 11, 2017August 19, 2017

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Recent Posts

  • Genome-wide libraries for CRISPRi (Dolcetto) and CRISPRa (Calabrese)!
  • Great links for making sense of TCR nomenclature!
  • “10 Best Science Podcasts About CRISPR”
  • Interesting study describing the transcriptional response of HSCs to CRISPR from the Porteus lab
  • “Accurate analysis of genuine CRISPR editing events with ampliCan”
  • Am I ready for CRISPR? A user’s guide to genetic screens by Dr. John G. Doench.
  • iBiology – A fantastic series of biology videos. Highly recommended!
  • Depmap (Broad Institute and collaborators) – Full genomic CRISPR screens >500 cancer cell lines (and counting).
  • I made a new video about how to look at FASTQ files from a CRISPR screen experiment using Excel (not recommended)
  • The phosphatase treatment of IVT sgRNAs is a cost-effective method for making highly active sgRNAs, avoiding innate immune responses in human cells.

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