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The Wermeling lab at the Karolinska Institutet

– Research related to Autoimmunity, Cancer immunotherapy and CRISPR/Cas9

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Addgene just published a blog post I´ve written about how we work with custom CRISPR screens!

http://blog.addgene.org/custom-crispr-screens-the-green-listed-software

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Author fredrikwermelingPosted on July 11, 2017August 19, 2017

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Previous Previous post: New funding from the “Clas Groschinsky minnesfond”!
Next Next post: CRISPR screen identifies that CMTM6 regulates the expression of PD-1L and anti-tumor immunity!

Top Posts & Pages

  • Links
  • Welcome!
  • Team
  • TIDE --> Identification of Indel frequencies in a CRISPR targeted cell population
  • Fantastic resources from the X. Shirley Liu Lab, including CRISPR-FOCUS!

Recent Posts

  • Am I ready for CRISPR? A user’s guide to genetic screens by Dr. John G. Doench.
  • iBiology – A fantastic series of biology videos. Highly recommended!
  • Depmap (Broad Institute and collaborators) – Full genomic CRISPR screens >500 cancer cell lines (and counting).
  • I made a new video about how to look at FASTQ files from a CRISPR screen experiment using Excel (not recommended)
  • The phosphatase treatment of IVT sgRNAs is a cost-effective method for making highly active sgRNAs, avoiding innate immune responses in human cells.
  • Interesting new book – Data Science for Immunologist
  • Interesting video about Machine learning and CRISPR Bioinformatics
  • We are very grateful for the continuous support from the Swedish Rheumatism Association and the Stiftelsen Professor Nanna Svartz Fond
  • TIDE –> Identification of Indel frequencies in a CRISPR targeted cell population
  • The Broad Cancer Cell Line Encyclopedia!

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