We have deposited our favorite CRISPR screen plasmid @Addgene

It’s a version of LentiGuide-puro where Sudeepta Panda and Long Jiang added P2A-eGFP after PuroR, lac-mRFP in the stuffer, and a cloning site directly after the sgRNA scaffold.

The plasmid allows for both puro and eGFP selection and MOI determination. The lac-mRFP in the stuffer gives a simple visual quality control step when expanding in bacteria (red=stuffer, white=spacer successfully introduced). The cloning site allows for introducing e.g. UMIs.


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