Related to CRISPR and CRISPR based screens

General info /


gRNA design /


Screen papers /

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench et al. Nature Biotechnology 2016. link

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari et al. Nature Methods 2015. link

Identification and characterization of essential genes in the human genome. Wang et al. Science 2015. link

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al. Nature, 2015 link (gain-of-function screen!)

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Koike-Yusa et al. Nature Biotechnology 2014. link

Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al. Science 2014. link

Improved vectors and genome-wide libraries for CRISPR screening. Sanjana et al. Nature Methods 2014. link

Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al. Science 2014. link

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench et al. Nature Biotechnology 2014. link

RNA-Guided Human Genome Engineering via Cas9. Mali et al. Science 2013. link