Related to CRISPR and CRISPR based screens

General info /

https://www.addgene.org/crispr/

https://www.addgene.org/crispr/guide/

http://blog.addgene.org/genome-wide-screening-using-crispr/cas9

https://www.addgene.org/crispr/libraries/

http://dharmacon.gelifesciences.com/resources/webinars/crispr-cas9-shrna-sgrna-pooled-lentiviral-screening/

—–

gRNA design /

http://research.microsoft.com/en-us/projects/azimuth/

http://www.broadinstitute.org/rnai/public/analysis-tools/sgrna-design

https://crispr.med.harvard.edu/main/

https://chopchop.rc.fas.harvard.edu/

http://crispr.wustl.edu/

http://crispr.mit.edu/

http://bioconductor.org/packages/release/bioc/html/CRISPRseek.html

https://www.dna20.com/eCommerce/cas9/input

—-

Screen papers /

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench et al. Nature Biotechnology 2016. link

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari et al. Nature Methods 2015. link

Identification and characterization of essential genes in the human genome. Wang et al. Science 2015. link

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al. Nature, 2015 link (gain-of-function screen!)

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Koike-Yusa et al. Nature Biotechnology 2014. link

Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al. Science 2014. link