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The Wermeling lab at the Karolinska Institutet

– Research related to Autoimmunity, Cancer immunotherapy and CRISPR/Cas9

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The RNAi Consortium (TRC)

Good information about shRNA design and screen:

The RNAi Consortium (TRC)

https://portals.broadinstitute.org/gpp/public/ 

https://www.nature.com/nmeth/journal/v3/n9/full/nmeth924.html

 

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Author fredrikwermelingPosted on October 15, 2017

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Previous Previous post: A hyper-accurate Cas9 variant from the Doudna lab!
Next Next post: “Guidelines for the use of flow cytometry and cell sorting in immunological studies.” – a great resource made up by 34 tables, 115 figures and 945 references!

Top Posts & Pages

  • Links
  • Depmap (Broad Institute and collaborators) - Full genomic CRISPR screens >500 cancer cell lines (and counting).
  • CRISPR screen identifies that CMTM6 regulates the expression of PD-1L and anti-tumor immunity!
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Recent Posts

  • New video – Generating a read count file from your custom CRISPR screen using Greenlisted and CRISPRAnalyzeR
  • Genome-wide libraries for CRISPRi (Dolcetto) and CRISPRa (Calabrese)!
  • Great links for making sense of TCR nomenclature!
  • “10 Best Science Podcasts About CRISPR”
  • Interesting study describing the transcriptional response of HSCs to CRISPR from the Porteus lab
  • “Accurate analysis of genuine CRISPR editing events with ampliCan”
  • Am I ready for CRISPR? A user’s guide to genetic screens by Dr. John G. Doench.
  • iBiology – A fantastic series of biology videos. Highly recommended!
  • Depmap (Broad Institute and collaborators) – Full genomic CRISPR screens >500 cancer cell lines (and counting).
  • I made a new video about how to look at FASTQ files from a CRISPR screen experiment using Excel (not recommended)

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