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Nucleosomes block access of CRISPR/Cas9 to DNA – Important study from the Weissman lab

Nucleosomes impede Cas9 access to DNA in vivo and in vitro.

Horlbeck MA, Witkowsky LB, Guglielmi B, Replogle JM, Gilbert LA, Villalta JE, Torigoe SE, Tjian R, Weissman JS.

Elife. 2016 Mar 17;5. pii: e12677. doi: 10.7554/eLife.12677.

PMID: 26987018
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Unknown's avatarAuthor fredrikwermelingPosted on January 15, 2017January 15, 2017

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  • Welcome!
  • Genome-wide libraries for CRISPRi (Dolcetto) and CRISPRa (Calabrese)!
  • Popular scientific texts, interviews, and outreach
  • New CRISPR screen analysis web app!
  • Very useful review - "Resources for the design of CRISPR gene editing experiments."

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  • New CRISPR screen analysis web app!
  • The “Analyzing CRISPR screens without coding experience” video series.
  • Green Listed v2.0 is now live!
  • Our CRISPR symposium was a success!
  • Tutorial: design and execution of CRISPR in vivo screens
  • One of our plasmids has recieved a Blue Flame Award from Addgene!
  • Bioicons – free science illustrations
  • Vaish has nailed her thesis!
  • New paper by Vaish!
  • I´m incredibly grateful for the Cancerfonden Senior Investigator Award!

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