–> http://crisprgold.mdc-berlin.de/
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Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.
Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kuhn R, Rajewsky K.
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519.
The Wermeling lab at the Karolinska Institutet 