Impressive CRISPR studies by Doench et al and Chari et al

These are two fantastic resources for guide design and CRISPR screens; taking both on-target and off-target activity into account when designing guides!

We are curious how the novel algorithms are going to perform related to modified Cas9 versions such as the eSpCas9(1.1) recently described by Slaymaker et al. (Science 2016. link).

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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench et al. Nature Biotechnology 2016. link

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari et al. Nature Methods 2015. link

 

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